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Biomolecules
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Small Molecules (<2000 mw) |
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| Discovery BIO GFC New! |
Discovery BIO GFC phases perform size exclusion separations over a wide molecular weight range, from small biomolecules to virus particles. By eliminating non-specific adsorption, the unique hydrophilic surface treatment and ultra high-purity silica combine to allow reproducibly high recovery of active proteins. Discovery BIO GFC is available in 5 µm spherical particles with pore sizes from 100 to 2000 Å for high separation capacity. The narrow pore and particle size distributions ensure high column efficiency and reproducibility.
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| Discovery BIO PolyMA-SCX |
| Efficient, high-recovery polymer-based cation-exchange columns for protein and peptide separations. |
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| Discovery BIO PolyMA-WAX |
| Efficient, high-recovery polymer-based anion-exchange columns for protein and peptide separations. |
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| Discovery BIO Wide Pore C5 |
Similar retention but increased stability compared to C4 with excellent, no bleed properties for LC-MS. Used for proteins and hydrophobic peptides.
Now in 0.18mm ID dimensions. |
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| Discovery BIO Wide Pore C8 |
| Intermediate hydrophobicity between a C4/C5 and C18 makes it a great starting point for peptide method development. Used for peptides and small proteins. |
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| Discovery BIO Wide Pore C18 |
The most hydrophobic BIO Wide Pore phase. Excellent for synthetic and natural peptides and peptide mapping.
Now in 0.18mm ID dimensions. |
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| Literature |
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Technical Bulletin:
Peptide Separations on a Stable, Aqueous-Compatible C18 Column
Most C18 HPLC columns undergo phase collapse in 100% aqueous mobile phases with concomitant loss of retention. This causes a problem when trying to analyze very polar compounds. This Technical Bulletin describes how Discovery BIO Wide Pore C18 columns do not undergo phase collapse, and can be used in 100% aqueous conditions. The implications for polar peptides and peptides mapping is described.
(52KB pdf) |
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Discovery BIO Wide Pore Brochure
Click to download the 36-page Discovery BIO Wide Pore brochure (9,866 KB pdf) |
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Getting Started with RP-HPLC for Peptides
Application Note 166 (69 KB pdf) |
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Increase Sensitivity and Decrease Sample Consumption Using HPLC Microbore and Capillary Column Dimensions
Application Note 167 (53 KB pdf) |
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Eliminate TFA and Improve Sensitivity of Peptide Analyses by LC-MS
Application Note 168 ( 58 KB pdf) |
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Effect of Stationary Phase on Selectivity of Reversed-Phase HPLC Separations of Polypeptides
Application Note 169 (75 KB pdf) |
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Short, Fast LC-MS Columns for Discovery Phases - C8, C18, cyano and HS F5 now available!
(more information) |
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| Discovery C18 and C8 NEW sizes available! |
| Classic reversed-phase selectivity and retention with exceptional peak shape for all compounds. Excellent, no-bleed properties for LC-MS applications. |
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| Discovery HS C18 |
| Non-polar, reversed-phase column with excellent, no bleed LC-MS performance |
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| Discovery RP-Amide C16 |
| Polar-embedded, palmitamidopropyl reversed-phase column with unique retention and selectivity. |
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| Discovery HS F5 NEW sizes available! |
Pentafluorophenyl terminated reversed-phase column with unique retention and selectivity
(e.g. basic & halogenated compounds) |
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| Discovery Cyano NEW sizes available! |
| Cyanopropyl reversed-phase column with lower hydrophobicity than C18 or C8 and unique selectivity. |
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| Discovery HS PEG |
Polyethylene glycol reversed-phase column with unique retention and selectivity
(e.g. phenolic compounds) |
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| Discovery Zr |
| For HPLC methods at pH and temperature extremes. |
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| Discovery Zr-PBD |
| Polybutadiene-coated zirconia particles for operation at pH 1-13 and up to 100°C (150°C in special hardware). Similar selectivity to C18-silica, but with pH and thermal stability. |
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| Discovery Zr-PS |
| Polystyrene-coated zirconia particles for operation at pH 1-13 and up to 100°C (150°C in special hardware). |
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| Discovery Zr-Carbon |
| Carbon-clad zirconia for separating isomers. Operates from pH 1-14 and up to 100°C (200°C in special hardware). |
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| Discovery Zr-CarbonC18 |
| C18-bonded carbon-clad zirconia combines shape selectivity with partitioning. Operates from pH 1-14 and up to 100°C (200°C in special hardware). |
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| Literature |
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Discovery HPLC Columns for Small Molecule Separations
Rediscover HPLC method development with this 76-page brochure, a practical tutorial that describes how Discovery allows you to develop better HPLC methods and solve common HPLC problems. Contains method development case studies, guidelines to choosing a Discovery column based on analyte and separation problem. Also contains information on the new Discovery Zr products. (1,1251 KB pdf) |
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Discovery Zr Method Development
Bulletin 932 (48 KB pdf) |
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